Polyphasic Analysis Occasionally fatty acid analysis and DNA sequencing can individually yield ambiguous or no-match results for identification. In such cases polyphasic analysis - combined fatty acid and DNA sequencing analysis - can provide additional information and produce a definitive identification. The key benefits of polyphasic analysis are: Confirmation of a definitive match resulting from one technique. Resolution of a non-match; a second technique can yield a definitive match in cases where the first technique gives no identification. Resolution of an inconclusive match; a second technique can yield a definitive match in cases where the first technique gives multiple possible identifications.